Heating device for 96-well microtiter culture plates

Thomas Bruns; Christian Berchtold; Herbert Schneckenburger

doi:10.1117/12.872859

11 February 2011 Heating device for 96-well microtiter culture plates

Thomas Bruns, Christian Berchtold, Herbert Schneckenburger

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 79021T (2011) https://doi.org/10.1117/12.872859
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

A heating device is presented, which can be attached on top of a standard microtiter plate, permitting individual and fast heating of the specimen by resistor elements without direct contact. All wells of the microtiter plate remain accessible from above and beyond for sensory (e.g. optical) measurements, independently from the bottom geometry of the wells. By various circuit configurations it is possible to realize either homogenous heating all over the plate or heating of individual arrays or clusters of wells, e.g. for maintaining defined temperature gradients. Main applications include quantitative measurements of temperature dependent parameters in the area of diagnostics, serology, cell culture- and immunological research. The heating device is validated by various referencing temperature kinetics and infrared images, as well as by selective investigations of cell membranes upon total internal reflection of a laser beam. For this purpose, particularly with regard to potential pharmaceutical applications, fluorescence decay kinetics of cancer cells incubated with temperature-dependent dyes, e.g. 6-dodecanoyl-2-dimethylaminonaphthalene (laurdan), is determined.

Citation Download Citation

Thomas Bruns, Christian Berchtold, and Herbert Schneckenburger "Heating device for 96-well microtiter culture plates", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021T (11 February 2011); https://doi.org/10.1117/12.872859

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