Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Heating device for 96-well microtiter culture plates

[+] Author Affiliations
Thomas Bruns, Christian Berchtold, Herbert Schneckenburger

Hochschule Aalen (Germany)

Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021T (February 10, 2011); doi:10.1117/12.872859
Text Size: A A A
From Conference Volume 7902

  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
  • Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif
  • San Francisco, California, USA | January 22, 2011

abstract

A heating device is presented, which can be attached on top of a standard microtiter plate, permitting individual and fast heating of the specimen by resistor elements without direct contact. All wells of the microtiter plate remain accessible from above and beyond for sensory (e.g. optical) measurements, independently from the bottom geometry of the wells. By various circuit configurations it is possible to realize either homogenous heating all over the plate or heating of individual arrays or clusters of wells, e.g. for maintaining defined temperature gradients. Main applications include quantitative measurements of temperature dependent parameters in the area of diagnostics, serology, cell culture- and immunological research. The heating device is validated by various referencing temperature kinetics and infrared images, as well as by selective investigations of cell membranes upon total internal reflection of a laser beam. For this purpose, particularly with regard to potential pharmaceutical applications, fluorescence decay kinetics of cancer cells incubated with temperature-dependent dyes, e.g. 6-dodecanoyl-2-dimethylaminonaphthalene (laurdan), is determined.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Thomas Bruns ; Christian Berchtold and Herbert Schneckenburger
"Heating device for 96-well microtiter culture plates", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021T (February 10, 2011); doi:10.1117/12.872859; http://dx.doi.org/10.1117/12.872859


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.