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Proceedings Article

Differentiating human cervical dysplastic and normal tissue through wavelet domain characterization of intrinsic fluorescence

[+] Author Affiliations
Rajatesh Gudibande

GMR Institute of Technology (India)

Meghdoot Mozumder, Rajbeer Singh, Asima Pradhan

Indian Institute of Technology Kanpur (India)

Prasanta K. Panigrahi

Indian Institute of Science Education and Research Kolkata (India)

Sharad Gupta

Univ. of California, Riverside (USA)

Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790220 (February 22, 2011); doi:10.1117/12.875246
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From Conference Volume 7902

  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
  • Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif
  • San Francisco, California, USA | January 22, 2011

abstract

Wavelet Transform based multi-resolution analysis has been used to characterize the intrinsic fluorescence of both dysplastic and normal human cervical tissues. The fluorescence spectra corresponding to 325nm and 370nm excitation from cervical dysplastic tissues of 48 patients from diverse age groups are studied in detail using Morlet wavelet. The wavelet modulus maxima lines for 325nm excitation indicated a distinct shift for dysplastic tissues towards the lower wavelengths with respect to normal ones. For 370nm excitation however, the shift for dysplastic tissues were towards the higher wavelengths. Sensitivities of 72% and 81% for spectral shifts of 325 and 370nm excitations were observed in the wavelength band of 425-500nm of the intensity spectra.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Rajatesh Gudibande ; Meghdoot Mozumder ; Rajbeer Singh ; Prasanta K. Panigrahi ; Sharad Gupta, et al.
"Differentiating human cervical dysplastic and normal tissue through wavelet domain characterization of intrinsic fluorescence", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790220 (February 22, 2011); doi:10.1117/12.875246; http://dx.doi.org/10.1117/12.875246


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