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Proceedings Article

3D myofibril imaging in live cardiomyocytes via hybrid SHG-TPEF microscopy

[+] Author Affiliations
Yonghong Shao, Junle Qu, Xiang Peng, Hanben Niu

Shenzhen Univ. (China)

Honghai Liu, Bruce Gao

Clemson Univ. (USA)

Tong Ye

Univ. of Alabama at Birmingham (USA)

Tom Borg

Medical Univ. of South Carolina (USA)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030F (March 03, 2011); doi:10.1117/12.875607
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We developed a hybrid SHG-TPEF polarization imaging system that allowed the excitation beam from an fs Ti:Sappire laser being bi-directionally raster scanned across the focal plane using a pair of orthogonal galvanometers. To implement high-speed scanning, the turning regions of the triangular waves were smoothed by a custom-designed waveform. The SHG and TPEF signals from samples were recorded by two PMTs in the forward and backward direction. Using this imaging system, we obtained 3D images of the sarcomere structure via SHG and DiO-stained lipid membrane via TPEF in live cardiomyocytes isolated from neonatal and adult rats. The results demonstrated the potential applications of SHG and TPEF in the research of myofibrillogensis.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Yonghong Shao ; Honghai Liu ; Tong Ye ; Tom Borg ; Junle Qu, et al.
"3D myofibril imaging in live cardiomyocytes via hybrid SHG-TPEF microscopy", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030F (March 03, 2011); doi:10.1117/12.875607; http://dx.doi.org/10.1117/12.875607


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