3D myofibril imaging in live cardiomyocytes via hybrid SHG-TPEF microscopy

Yonghong Shao; Honghai Liu; Tong Ye; Tom Borg; Junle Qu; Xiang Peng; Hanben Niu; Bruce Gao

doi:10.1117/12.875607

3 March 2011 3D myofibril imaging in live cardiomyocytes via hybrid SHG-TPEF microscopy

Yonghong Shao, Honghai Liu, Tong Ye, Tom Borg, Junle Qu, Xiang Peng, Hanben Niu, Bruce Gao

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79030F (2011) https://doi.org/10.1117/12.875607
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We developed a hybrid SHG-TPEF polarization imaging system that allowed the excitation beam from an fs Ti:Sappire laser being bi-directionally raster scanned across the focal plane using a pair of orthogonal galvanometers. To implement high-speed scanning, the turning regions of the triangular waves were smoothed by a custom-designed waveform. The SHG and TPEF signals from samples were recorded by two PMTs in the forward and backward direction. Using this imaging system, we obtained 3D images of the sarcomere structure via SHG and DiO-stained lipid membrane via TPEF in live cardiomyocytes isolated from neonatal and adult rats. The results demonstrated the potential applications of SHG and TPEF in the research of myofibrillogensis.

Citation Download Citation

Yonghong Shao, Honghai Liu, Tong Ye, Tom Borg, Junle Qu, Xiang Peng, Hanben Niu, and Bruce Gao "3D myofibril imaging in live cardiomyocytes via hybrid SHG-TPEF microscopy", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030F (3 March 2011); https://doi.org/10.1117/12.875607

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