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Proceedings Article

Second harmonic phase microscopy of collagen fibers

[+] Author Affiliations
Etienne Shaffer, Christian Depeursinge

Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Pierre Marquet

Ecole Polytechnique Fédérale de Lausanne (Switzerland) and Ctr. Hospitalier Univ. Vaudois (Switzerland)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030G (February 10, 2011); doi:10.1117/12.874538
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional information, inaccessible to incoherent imaging schemes. Last year, we have reported how the SHG phase makes possible real-time nanometric 3D-tracking of SHG emitters, such as nanoparticles (Shaffer et al., Opt. Express 18, p.17392-17403, 2010). Here, we investigate the phase of second harmonic generated by a label-free biological specimen-more precisely collagen fibers forming the connective tissue of a mouse dermis- and discuss of its interpretation. Notably, we show how the SHG phase, qualitatively acting as an indicator of phase-matching conditions, tends to indicate that second harmonic generation, in collagen, is dominated by coherent SHG scattering.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Etienne Shaffer ; Pierre Marquet and Christian Depeursinge
"Second harmonic phase microscopy of collagen fibers", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030G (February 10, 2011); doi:10.1117/12.874538; http://dx.doi.org/10.1117/12.874538


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