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Proceedings Article

Combining multiphoton and CARS microscopy for skin imaging

[+] Author Affiliations
H. G. Breunig, M. Weinigel

JenLab GmbH (Germany)

J. Lademann, W. Sterry

Charité (Germany)

I. Latka, B. Dietzek, J. Popp

IPHT, Univ. of Jena (Germany)

K. König

JenLab GmbH (Germany) and Saarland Univ. (Germany)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031A (February 10, 2011); doi:10.1117/12.874969
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We present combined coherent anti Stokes Raman scattering and two-photon excited fluorescence microscopy, for label-free in vitro and in vivo imaging of skin with both chemical discrimination and subcellular resolution. In particular, we show how CARS microscopy is utilized to image lipid-rich structures inside the skin, preparing for a combined multiphoton and CARS imaging modality for biomedical research and skin imaging. As a further potential application the detection of topically applied oil on the skin is presented.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

H. G. Breunig ; M. Weinigel ; J. Lademann ; W. Sterry ; I. Latka, et al.
"Combining multiphoton and CARS microscopy for skin imaging", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031A (February 10, 2011); doi:10.1117/12.874969; http://dx.doi.org/10.1117/12.874969


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