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Proceedings Article

Coherent anti-Stokes Raman scattering imaging through turbid medium

[+] Author Affiliations
Rajan Arora, Georgi I. Petrov, Vladislav V. Yakovlev

Univ. of Wisconsin-Milwaukee (USA)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031B (February 28, 2011); doi:10.1117/12.875433
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

Coherent Raman microspectroscopy imaging is an emerging technique for non-invasive, chemically-specific optical imaging. The wealth of information available through hyperspectral imaging potentially allows discriminating and imaging different chemicals in biological tissues, which is important, for example, for stain-free histopathology. One of the advantages of nonlinear Raman microspectroscopy is the ability to achieve high spatial resolution imaging in the presence of light scattering, when conventional confocal microscopy fails. In this report, a commercially available hyperspectral analysis software is utilized for analysis of vibrational spectra collected through highly scattering medium In particular, applications to remote optical sensing of potential biological threats and to imaging through a layer of skin tissue were successfully demonstrated.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Rajan Arora ; Georgi I. Petrov and Vladislav V. Yakovlev
"Coherent anti-Stokes Raman scattering imaging through turbid medium", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031B (February 28, 2011); doi:10.1117/12.875433; http://dx.doi.org/10.1117/12.875433


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