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Proceedings Article

Differential CARS microscopy with linearly chirped femtosecond laser pulses

[+] Author Affiliations
Wolfgang Langbein, Israel Rocha-Mendoza, Francesco Masia, Claudia Di Napoli, Iestyn Pope, Peter Watson, Paola Borri

Cardiff Univ. (United Kingdom)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031I (February 10, 2011); doi:10.1117/12.873872
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We demonstrate frequency differential CARS (D-CARS) using femtosecond laser pulses linearly chirped by glass elements of high group-velocity dispersion. By replicating the Pump-Stokes pair into a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference by glass dispersion, we adjust the Raman frequency probed by each pair in an intrinsically stable way. The resulting CARS intensities are detected simultaneously by a single photomultiplier as sum and difference using lock-in detection. We demonstrate imaging of living cells with strongly suppressed non-resonant background. We also show D-CARS using a single femtosecond laser source.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Wolfgang Langbein ; Israel Rocha-Mendoza ; Francesco Masia ; Claudia Di Napoli ; Iestyn Pope, et al.
"Differential CARS microscopy with linearly chirped femtosecond laser pulses", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031I (February 10, 2011); doi:10.1117/12.873872; http://dx.doi.org/10.1117/12.873872


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