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Proceedings Article

Wide-field two-photon microscopy with temporal focusing and HiLo background rejection

[+] Author Affiliations
Elijah Y. S. Yew

Singapore-MIT Alliance for Research and Technology (Singapore)

Heejin Choi, Daekeun Kim

Massachusetts Institute of Technology (USA)

Peter T. C. So

Singapore-MIT Alliance for Research and Technology (Singapore) and Massachusetts Institute of Technology (USA)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031O (February 28, 2011); doi:10.1117/12.876068
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

Scanningless depth-resolved microscopy is achieved through spatial-temporal focusing and has been demonstrated previously. The advantage of this method is that a large area may be imaged without scanning resulting in higher throughput of the imaging system. Because it is a widefield technique, the optical sectioning effect is considerably poorer than with conventional spatial focusing two-photon microscopy. Here we propose wide-field two-photon microscopy based on spatio-temporal focusing and employing background rejection based on the HiLo microscope principle. We demonstrate the effects of applying HiLo microscopy to widefield temporally focused two-photon microscopy.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Elijah Y. S. Yew ; Heejin Choi ; Daekeun Kim and Peter T. C. So
"Wide-field two-photon microscopy with temporal focusing and HiLo background rejection", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031O (February 28, 2011); doi:10.1117/12.876068; http://dx.doi.org/10.1117/12.876068


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