Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Raman spectroscopy: a powerful tool for the non-contact discrimination of bone marrow mesenchymal stem cells and fibroblasts

[+] Author Affiliations
Marieke Pudlas, Steffen Koch, Thomas Hirth

Fraunhofer IGB (Germany) and Univ. of Stuttgart (Germany)

Carsten Bolwien

Fraunhofer IPM (Germany)

Heike Walles

Fraunhofer IGB (Germany) and Julius-Maximillians Univ. Würzburg (Germany)

Katja Schenke-Layland

Fraunhofer IGB (Germany)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032I (February 10, 2011); doi:10.1117/12.873047
Text Size: A A A
From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

Bone-marrow mesenchymal stem cells (BM-MSCs) are a promising cell source for regenerative medicine. However, it is challenging to determine whether isolated BM-MSC populations are free of fibroblasts. We employed traditional methods and Raman spectroscopy to distinguish BM-MSCs and human dermal fibroblasts (hDFs). Although in vitro differentiation assays revealed the multipotent character of BM-MSCs, long culture periods are a major disadvantage. Using Raman spectroscopy, we could quickly distinguish between BM-MSCs and hDFs. Therefore we conclude that this method is sufficient for the rapid detection of fibroblastic contaminations in BM-MSC cultures.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Marieke Pudlas ; Steffen Koch ; Carsten Bolwien ; Thomas Hirth ; Heike Walles, et al.
"Raman spectroscopy: a powerful tool for the non-contact discrimination of bone marrow mesenchymal stem cells and fibroblasts", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032I (February 10, 2011); doi:10.1117/12.873047; http://dx.doi.org/10.1117/12.873047


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.