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Proceedings Article

Broadband coherent Raman imaging for multiplexed detection

[+] Author Affiliations
Brad Littleton, Fred Festy, Simon Ameer-Beg, David Richards

King's College London (United Kingdom)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032J (February 28, 2011); doi:10.1117/12.875180
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

The Raman spectrum of a fluorescent chromophore typically has many spectral features, which differ markedly between dyes even if their electronic spectra are similar. This high information content makes it possible to distinguish biomarkers based on their Raman spectra. Coherent anti-Stokes Raman scattering may therefore allow for the simultaneous measurement of more biomarkers than is possible with fluorescent imaging, while avoiding bleaching and sample autofluorescence. We have built a broadband CARS microspectrometer to demonstrate the principle of CARS multiplexing and investigate the potential to apply the system to studies of biological samples.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Brad Littleton ; Fred Festy ; Simon Ameer-Beg and David Richards
"Broadband coherent Raman imaging for multiplexed detection", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032J (February 28, 2011); doi:10.1117/12.875180; http://dx.doi.org/10.1117/12.875180


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