Broadband coherent Raman imaging for multiplexed detection

Brad Littleton; Fred Festy; Simon Ameer-Beg; David Richards

doi:10.1117/12.875180

28 February 2011 Broadband coherent Raman imaging for multiplexed detection

Brad Littleton, Fred Festy, Simon Ameer-Beg, David Richards

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79032J (2011) https://doi.org/10.1117/12.875180
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The Raman spectrum of a fluorescent chromophore typically has many spectral features, which differ markedly between dyes even if their electronic spectra are similar. This high information content makes it possible to distinguish biomarkers based on their Raman spectra. Coherent anti-Stokes Raman scattering may therefore allow for the simultaneous measurement of more biomarkers than is possible with fluorescent imaging, while avoiding bleaching and sample autofluorescence. We have built a broadband CARS microspectrometer to demonstrate the principle of CARS multiplexing and investigate the potential to apply the system to studies of biological samples.

Citation Download Citation

Brad Littleton, Fred Festy, Simon Ameer-Beg, and David Richards "Broadband coherent Raman imaging for multiplexed detection", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032J (28 February 2011); https://doi.org/10.1117/12.875180

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