Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Diagnosing hepatocellular carcinoma with the intensity and the lifetime of two-photon red autofluorescences

[+] Author Affiliations
Tzu-Ming Liu, Chien-Tai Hsieh, Yu-Shing Chen, Fu-Lien Huang, Chun-Ta Kung, Chi-Kuang Sun

National Taiwan Univ. (Taiwan)

Hsin-Yi Huang, Wen-Jeng Lee

National Taiwan Univ. Hospital (Taiwan)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032Z (February 10, 2011); doi:10.1117/12.872690
Text Size: A A A
From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We demonstrated that the intensity levels and lifetimes of two-photon autofluorescences (2PAF) in human liver tissues can be exploited to diagnose hepatocellular carcinoma (HCC). Excited by an infrared femtosecond laser, we suppressed the two-photon autofluorescences of most endogenous fluorophores and made red autofluorescences more specific to particular molecules in the cryo-sectioned human livers. Using such fluorescence contrast, we found HCC tissues have reduced levels of 2PAF and longer fluorescence lifetime.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Tzu-Ming Liu ; Chien-Tai Hsieh ; Yu-Shing Chen ; Fu-Lien Huang ; Hsin-Yi Huang, et al.
"Diagnosing hepatocellular carcinoma with the intensity and the lifetime of two-photon red autofluorescences", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032Z (February 10, 2011); doi:10.1117/12.872690; http://dx.doi.org/10.1117/12.872690


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.