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Proceedings Article

Delivery and characterization of sub-8fs laser pulses at the imaging plane of a two-photon microscope

[+] Author Affiliations
Dmitry Pestov, Bingwei Xu, Haowen Li

Biophotonic Solutions, Inc. (USA)

Marcos Dantus

Biophotonic Solutions, Inc. (USA) and Michigan State Univ. (USA)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79033B (February 28, 2011); doi:10.1117/12.889223
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We demonstrate a modular and versatile experimental setup that enables straightforward compression (and then shaping) of ultrashort laser pulses at the imaging plane of a two-photon microscope. A commercially available pulse shaper is used in conjunction with a commercially available broadband Ti:Sapphire oscillator to produce sub-8fs pulses at the focus of a high-numerical-aperture objective. Automated adaptive pulse compression, based on multiphoton intrapulse interference phase scan (MIIPS), is verified in situ by shaper assisted interferometric autocorrelation. Two-photon excited fluorescence image of a mouse kidney slide is obtained to confirm microscopic sectioning capabilities.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Dmitry Pestov ; Bingwei Xu ; Haowen Li and Marcos Dantus
"Delivery and characterization of sub-8fs laser pulses at the imaging plane of a two-photon microscope", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79033B (February 28, 2011); doi:10.1117/12.889223; http://dx.doi.org/10.1117/12.889223


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