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Proceedings Article

Using multimodal femtosecond CARS imaging to determine plaque burden in luminal atherosclerosis

[+] Author Affiliations
Alex C-T. Ko, Leila B. Mostaço-Guidolin, Andrew Ridsdale, Adrian F. Pegoraro, Michael S. D. Smith, Aaron Slepkov, Mark D. Hewko, Elicia K. Kohlenberg, Bernie Schattka, Albert Stolow, Michael G. Sowa

National Research Council Canada (Canada)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790318 (February 22, 2011); doi:10.1117/12.873867
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

Luminal atherosclerosis imaging was demonstrated by multimodal femtosecond CARS microscopy (MM-CARS). Using a myocardial infarction-prone rabbit model of atherosclerosis, this study demonstrated the utility of multimodal CARS imaging in determining atherosclerotic plaque burden through two types of image analysis procedures. Firstly, multimodal CARS images were evaluated using a signal-intensity parameter based on intensity changes derived from the multi-channel data (e.g. TPEF, SHG and CARS) to classify plaque burden within the vessel. Secondly, the SHG images that mainly correspond to collagen fibrils were evaluated using a texture analysis model based on the first-order statistical (FOS) parameters of the image histogram. Correlation between FOS parameters of collagen images with atherosclerosis plaque burden was established. A preliminary study of using spectroscopic CARS in identifying the different lipid components within the plaque was also discussed.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Alex C-T. Ko ; Leila B. Mostaço-Guidolin ; Andrew Ridsdale ; Adrian F. Pegoraro ; Michael S. D. Smith, et al.
"Using multimodal femtosecond CARS imaging to determine plaque burden in luminal atherosclerosis", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790318 (February 22, 2011); doi:10.1117/12.873867; http://dx.doi.org/10.1117/12.873867


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