Determination of calcium concentrations in cells and tissue with fluorescence lifetime imaging (FILM)

T. Gensch; M. Wirth

doi:10.1117/12.874507

28 February 2011 Determination of calcium concentrations in cells and tissue with fluorescence lifetime imaging (FILM)

T. Gensch, M. Wirth

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 790322 (2011) https://doi.org/10.1117/12.874507
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The determination of ion concentrations in cells - in particular in neurons - is very important for understanding cell function and life. Calcium is an ubiquitous messenger in almost all cell types. Fluorescence lifetime imaging (FLIM) can be of advantage over intensity based fluorescence microscopy, when comparisons between micro-domains of one cell or between different cells of one cell type are performed. Several organic chromophores have been tested in cuvette experiments as well as in living cells and cell tissue with respect to their applicability in FLIM studies. The calcium concentration changes in several cell types were investigated by FLIM with two-photon excitation.

Citation Download Citation

T. Gensch and M. Wirth "Determination of calcium concentrations in cells and tissue with fluorescence lifetime imaging (FILM)", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790322 (28 February 2011); https://doi.org/10.1117/12.874507

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