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Proceedings Article

Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells

[+] Author Affiliations
Pei-Hua Chung, James A. Levitt, Klaus Suhling

King's College London (United Kingdom)

Marina K. Kuimova

Imperial College London (United Kingdom)

Gokhan Yahioglu

Imperial College London (United Kingdom) and PhotoBiotics Ltd. (United Kingdom)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790323 (February 22, 2011); doi:10.1117/12.876257
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

Meso-substituted boron-dipyrromethene (BODIPY-C12) was used to monitor the viscosity in cells via fluorescence lifetime imaging (FLIM), and time-resolved fluorescence anisotropy measurements. Our results show that meso-substituted BODIPY-C12 senses the viscosity in HeLa cells and is insensitive to the surrounding polarity. The relationship between the fluorescence lifetime and the rotational correlation time of the dye in homogeneous solutions agree with the combination of the Foerster Hoffmann equation and the Debye-Stokes-Einstein equation.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Pei-Hua Chung ; James A. Levitt ; Marina K. Kuimova ; Gokhan Yahioglu and Klaus Suhling
"Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790323 (February 22, 2011); doi:10.1117/12.876257; http://dx.doi.org/10.1117/12.876257


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