Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells

Pei-Hua Chung; James A. Levitt; Marina K. Kuimova; Gokhan Yahioglu; Klaus Suhling

doi:10.1117/12.876257

22 February 2011 Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells

Pei-Hua Chung, James A. Levitt, Marina K. Kuimova, Gokhan Yahioglu, Klaus Suhling

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 790323 (2011) https://doi.org/10.1117/12.876257
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Meso-substituted boron-dipyrromethene (BODIPY-C₁₂) was used to monitor the viscosity in cells via fluorescence lifetime imaging (FLIM), and time-resolved fluorescence anisotropy measurements. Our results show that meso-substituted BODIPY-C₁₂ senses the viscosity in HeLa cells and is insensitive to the surrounding polarity. The relationship between the fluorescence lifetime and the rotational correlation time of the dye in homogeneous solutions agree with the combination of the Foerster Hoffmann equation and the Debye-Stokes-Einstein equation.

Citation Download Citation

Pei-Hua Chung, James A. Levitt, Marina K. Kuimova, Gokhan Yahioglu, and Klaus Suhling "Mapping intracellular viscosity by advanced fluorescence imaging of molecular rotors in living cells", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790323 (22 February 2011); https://doi.org/10.1117/12.876257

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available