Two-photon excitation in life sciences: neurotransmitter and fluorescence uncaging

Frédéric Bolze; Jean-François Nicoud; Sylvestre Gug; Sébastien Charon; Alexandre Specht; Maurice Goeldner; David Warther; Xiao-Hua Sun; Pascal Kessler; Yves Lutz; Jean-Luc Vonesch; Attila Losonczy

doi:10.1117/12.873742

22 February 2011 Two-photon excitation in life sciences: neurotransmitter and fluorescence uncaging

Frédéric Bolze, Jean-François Nicoud, Sylvestre Gug, Sébastien Charon, Alexandre Specht, Maurice Goeldner, David Warther, Xiao-Hua Sun, Pascal Kessler, Yves Lutz, Jean-Luc Vonesch, Attila Losonczy

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 790339 (2011) https://doi.org/10.1117/12.873742
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We describe the molecular engineering of new highly efficient two-photon photolabile cages, based on dissymmetrical donor-acceptor and symmetrical donor-donor systems. Complete characterizations of these new cages, based on a biphenyl, fluorenyl or a 2,5-diphenylthienyl central core will be presented. In vitro photochemistry will be described (uncaging quantum yield, two-photon uncaging action cross-section,...) and we will then focus on biological results obtained in cell culture and on acute brain slices (with glutamate and fluorescence uncaging).

Citation Download Citation

Frédéric Bolze, Jean-François Nicoud, Sylvestre Gug, Sébastien Charon, Alexandre Specht, Maurice Goeldner, David Warther, Xiao-Hua Sun, Pascal Kessler, Yves Lutz, Jean-Luc Vonesch, and Attila Losonczy "Two-photon excitation in life sciences: neurotransmitter and fluorescence uncaging", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790339 (22 February 2011); https://doi.org/10.1117/12.873742

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