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Proceedings Article

Two-photon excitation in life sciences: neurotransmitter and fluorescence uncaging

[+] Author Affiliations
Frédéric Bolze, Jean-François Nicoud, Xiao-Hua Sun

Lab. de Biphotonique et Pharmacologie, CNRS, Univ. de Strasbourg (France)

Sylvestre Gug, Sébastien Charon

Lab. de Biphotonique et Pharmacologie, CNRS, Univ. de Strasbourg (France) and Lab. de Conception et Application de Molécules Bioactives, CNRS, Univ. de Strasbourg (France)

Alexandre Specht, Maurice Goeldner, David Warther

Lab. de Conception et Application de Molécules Bioactives, CNRS, Univ. de Strasbourg (France)

Pascal Kessler, Yves Lutz, Jean-Luc Vonesch

Institut de Genetique et Biologie Moleculaire et Cellulaire (France)

Attila Losonczy

Columbia Univ. (USA)

Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790339 (February 22, 2011); doi:10.1117/12.873742
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From Conference Volume 7903

  • Multiphoton Microscopy in the Biomedical Sciences XI
  • Ammasi Periasamy; Karsten König; Peter T. C. So
  • San Francisco, California, USA | January 22, 2011

abstract

We describe the molecular engineering of new highly efficient two-photon photolabile cages, based on dissymmetrical donor-acceptor and symmetrical donor-donor systems. Complete characterizations of these new cages, based on a biphenyl, fluorenyl or a 2,5-diphenylthienyl central core will be presented. In vitro photochemistry will be described (uncaging quantum yield, two-photon uncaging action cross-section,...) and we will then focus on biological results obtained in cell culture and on acute brain slices (with glutamate and fluorescence uncaging).

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Frédéric Bolze ; Jean-François Nicoud ; Sylvestre Gug ; Sébastien Charon ; Alexandre Specht, et al.
"Two-photon excitation in life sciences: neurotransmitter and fluorescence uncaging", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790339 (February 22, 2011); doi:10.1117/12.873742; http://dx.doi.org/10.1117/12.873742


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