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Proceedings Article

3D optical trapping calibration and optical micromanipulation using 808-nm diode-laser bar

[+] Author Affiliations
Mariana Potcoava

Colorado School of Mines (USA) and JILA, Univ. of Colorado and National Institute of Standards and Technology (USA)

Leo Krzewina, Myung K. Kim

Univ. of South Florida (USA)

Erich Hoover, Jeff Squier, David W. M. Marr

Colorado School of Mines (USA)

Ralph Jimenez

JILA, Univ. of Colorado and National Institute of Standards and Technology (USA)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040A (February 10, 2011); doi:10.1117/12.876010
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

It has recently been demonstrated that diode laser bars can be used to not only optically trap red blood cells in flowing microfluidic systems but also, stretch, bend, and rotate them. To predict the complex cell behavior at different locations along a linear trap, 3D optical force characterization is required. The driving force for cells or colloidal particles within an optical trap is the thermal Brownian force where particle fluctuations can be considered a stochastic process. For optical force quantification, we combine diode laser bar optical trapping with Gabor digital holography imaging to perform subpixel resolution measurements of micron-sized particles positions along the laser bar. Here, diffraction patterns produced by trapped particles illuminated by a He-Ne laser are recorded with a CMOS sensor at 1000 fps where particle beam position reconstruction is performed using the angular spectrum method and centroid position detection. 3D optical forces are then calculated by three calibration methods: the equipartition theorem, Boltzmann probability distribution, and power spectral density analysis for each particle in the trap. This simple approach for 3D tracking and optical control can be implemented on any transmission microscope by adding a laser beam as the illumination source instead of a white light source.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Mariana Potcoava ; Leo Krzewina ; Erich Hoover ; Myung K. Kim ; Jeff Squier, et al.
"3D optical trapping calibration and optical micromanipulation using 808-nm diode-laser bar", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040A (February 10, 2011); doi:10.1117/12.876010; http://dx.doi.org/10.1117/12.876010


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