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Proceedings Article

High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens

[+] Author Affiliations
Shau Poh Chong, Guangjun Gao, Colin J. R. Sheppard, Nanguang Chen

National Univ. of Singapore (Singapore)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040C (February 10, 2011); doi:10.1117/12.873996
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Shau Poh Chong ; Guangjun Gao ; Colin J. R. Sheppard and Nanguang Chen
"High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040C (February 10, 2011); doi:10.1117/12.873996; http://dx.doi.org/10.1117/12.873996


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