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Proceedings Article

Quadriwave lateral shearing interferometry for quantitative phase microscopy: coupling phase imaging and fluorescence imaging

[+] Author Affiliations
Pierre Bon

Institut Fresnel, CNRS, Aix-Marseille Univ. (France) and PHASICS S.A. (France)

Julien Savatier, Serge Monneret

Institut Fresnel, CNRS, Aix-Marseille Univ. (France)

Benoit Wattellier

PHASICS S.A. (France)

Didier Marguet

Ctr. Immunologique de Marseille Luminy, CNRS, Univ. de Provence (France)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040P (February 22, 2011); doi:10.1117/12.873177
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

Phase imaging with a high-resolution wavefront sensor is a useful setup for biological imaging. Our setup is based on a quadriwave lateral shearing interferometer mounted on a commercial non-modified transmission white-light microscope. That allows us to make simultaneous measurement in both quantitative transmission phase and fluorescence imaging. We propose here to study co-localization between phase and fluorescence on african green monkey kidney COS7 cells. Phase permits an enhanced visualization of the whole cell and intracellular components while the fluorescence allows a complete identification of each component. Post treatments on phase-shift images are proposed and become very interesting for enhanced visualization of small details such as vesicles or mitochondrias.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Pierre Bon ; Julien Savatier ; Benoit Wattellier ; Didier Marguet and Serge Monneret
"Quadriwave lateral shearing interferometry for quantitative phase microscopy: coupling phase imaging and fluorescence imaging", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040P (February 22, 2011); doi:10.1117/12.873177; http://dx.doi.org/10.1117/12.873177


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