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Proceedings Article

Optically sectioned imaging by oblique plane microscopy

[+] Author Affiliations
Sunil Kumar, Ziduo Lin, Alex R. Lyon, Ken T. MacLeod, Chris Dunsby

Imperial College London (United Kingdom)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040X (February 28, 2011); doi:10.1117/12.873471
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

Oblique Plane Microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. The first OPM results obtained using a high NA water immersion lens on a commercially available inverted microscope frame are presented, together with a measurement of the achievable optical resolution.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Sunil Kumar ; Ziduo Lin ; Alex R. Lyon ; Ken T. MacLeod and Chris Dunsby
"Optically sectioned imaging by oblique plane microscopy", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040X (February 28, 2011); doi:10.1117/12.873471; http://dx.doi.org/10.1117/12.873471


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