Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

An automated wide-field time-gated optically sectioning fluorescence lifetime imaging multiwell plate reader for high-content analysis of protein-protein interactions

[+] Author Affiliations
Dominic Alibhai, Sunil Kumar, Douglas Kelly, Sean Warren, Yuriy Alexandrov, Ian Munro, James McGinty, Clifford Talbot, Mark A. A. Neil, Chris Dunsby, Paul M. W. French

Imperial College London (United Kingdom)

Edward J. Murray, Frank Stuhmeier

Pfizer Group Ltd. (United Kingdom)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79041Q (February 28, 2011); doi:10.1117/12.875135
Text Size: A A A
From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

We describe an optically-sectioned FLIM multiwell plate reader that combines Nipkow microscopy with wide-field time-gated FLIM, and its application to high content analysis of FRET. The system acquires sectioned FLIM images in <10 s/well, requiring only ~11 minutes to read a 96 well plate of live cells expressing fluorescent protein. It has been applied to study the formation of immature HIV virus like particles (VLPs) in live cells by monitoring Gag-Gag protein interactions using FLIM FRET of HIV-1 Gag transfected with CFP or YFP. VLP formation results in FRET between closely packed Gag proteins, as confirmed by our FLIM analysis that includes automatic image segmentation.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Dominic Alibhai ; Sunil Kumar ; Douglas Kelly ; Sean Warren ; Yuriy Alexandrov, et al.
"An automated wide-field time-gated optically sectioning fluorescence lifetime imaging multiwell plate reader for high-content analysis of protein-protein interactions", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79041Q (February 28, 2011); doi:10.1117/12.875135; http://dx.doi.org/10.1117/12.875135


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.