Three-dimensional data acquisition with aberrations correction for video-rate microscopy

Masood Samim; Richard Cisek; Daaf Sandkuijl; Ian Tretyakov; Sam Siu; Sergey Musikhin; Virginijus Barzda

doi:10.1117/12.875907

23 February 2011 Three-dimensional data acquisition with aberrations correction for video-rate microscopy

Masood Samim, Richard Cisek, Daaf Sandkuijl, Ian Tretyakov, Sam Siu, Sergey Musikhin, Virginijus Barzda

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 790413 (2011) https://doi.org/10.1117/12.875907
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We demonstrate a multimodal, multifocal, differential nonlinear optical microscope, which is equipped with a pair of deformable mirrors and a Shack-Hartmann sensor for dynamic wavefront manipulation. The optical wavefronts of a home built Yb:KGW femtosecond (1028 nm) laser-beams are engineered to perform multidepth focusing in differential mode with simultaneous corrections for optical aberrations. The 39-actuator deformable mirrors provide fast reshaping of the wavefront and optical aberrations correction of the diffraction-limited focal volume allowing for fast axial scanning. Combination of ~200 frames per second lateral scanning with fast refocusing enables a three-dimensional video rate scanning capability, which is essential for studying rapid dynamics in biological organisms, such as blood flow, cardiac contractions, and motility of microorganisms in a three-dimensional volume.

Citation Download Citation

Masood Samim, Richard Cisek, Daaf Sandkuijl, Ian Tretyakov, Sam Siu, Sergey Musikhin, and Virginijus Barzda "Three-dimensional data acquisition with aberrations correction for video-rate microscopy", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790413 (23 February 2011); https://doi.org/10.1117/12.875907

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