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Proceedings Article

Three-dimensional data acquisition with aberrations correction for video-rate microscopy

[+] Author Affiliations
Masood Samim, Richard Cisek, Daaf Sandkuijl, Ian Tretyakov, Sam Siu, Sergey Musikhin, Virginijus Barzda

Univ. of Toronto (Canada)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790413 (February 22, 2011); doi:10.1117/12.875907
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

We demonstrate a multimodal, multifocal, differential nonlinear optical microscope, which is equipped with a pair of deformable mirrors and a Shack-Hartmann sensor for dynamic wavefront manipulation. The optical wavefronts of a home built Yb:KGW femtosecond (1028 nm) laser-beams are engineered to perform multidepth focusing in differential mode with simultaneous corrections for optical aberrations. The 39-actuator deformable mirrors provide fast reshaping of the wavefront and optical aberrations correction of the diffraction-limited focal volume allowing for fast axial scanning. Combination of ~200 frames per second lateral scanning with fast refocusing enables a three-dimensional video rate scanning capability, which is essential for studying rapid dynamics in biological organisms, such as blood flow, cardiac contractions, and motility of microorganisms in a three-dimensional volume.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Masood Samim ; Richard Cisek ; Daaf Sandkuijl ; Ian Tretyakov ; Sam Siu, et al.
"Three-dimensional data acquisition with aberrations correction for video-rate microscopy", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790413 (February 22, 2011); doi:10.1117/12.875907; http://dx.doi.org/10.1117/12.875907


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