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Proceedings Article

Adaptive optics for wide-field microscopy

[+] Author Affiliations
C. Bourgenot, C. D. Saunter, J. M. Girkin, G. D. Love

Durham Univ. (United Kingdom)

Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790414 (February 22, 2011); doi:10.1117/12.873857
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From Conference Volume 7904

  • Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
  • Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown
  • San Francisco, California, USA | January 22, 2011

abstract

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

C. Bourgenot ; C. D. Saunter ; J. M. Girkin and G. D. Love
"Adaptive optics for wide-field microscopy", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790414 (February 22, 2011); doi:10.1117/12.873857; http://dx.doi.org/10.1117/12.873857


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