Fourier analysis of mitochondrial distribution in oocytes

Joseph L. Hollmann; Dana H. Brooks; Judith A. Newmark; Carol M. Warner; Charles A. DiMarzio

doi:10.1117/12.876345

23 February 2011 Fourier analysis of mitochondrial distribution in oocytes

Joseph L. Hollmann, Dana H. Brooks, Judith A. Newmark, Carol M. Warner, Charles A. DiMarzio

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 790419 (2011) https://doi.org/10.1117/12.876345
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

This paper describes a novel approach to quantifying mitochondrial patterns which are typically described using the qualitative terms "diffuse" "aggregated" and are potentially key indicators for an oocyte's health and survival potential post-implantation. An oocyte was isolated in a confocal image and a coarse grid was superimposed upon it. The spatial spectrum was calculated and an aggregation factor was generated. A classifier for healthy cells was developed and verified. The aggregation factor showed a clear distinction between the healthy and unhealthy oocytes. The ultimate goal is to screen oocytes for viability preimplantation, thus improving the outcome of in vitro fertilization (IVF) treatments.

Citation Download Citation

Joseph L. Hollmann, Dana H. Brooks, Judith A. Newmark, Carol M. Warner, and Charles A. DiMarzio "Fourier analysis of mitochondrial distribution in oocytes", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790419 (23 February 2011); https://doi.org/10.1117/12.876345

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