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Proceedings Article

Confocal imaging at the nanoscale with two-color STED microscopy

[+] Author Affiliations
Hilmar Gugel, Arnold Giske, Marcus Dyba, Jochen Sieber

Leica Microsystems CMS GmbH (Germany)

Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050X (February 28, 2011); doi:10.1117/12.874779
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From Conference Volume 7905

  • Single Molecule Spectroscopy and Imaging IV
  • Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann
  • San Francisco, California, USA | January 22, 2011

abstract

STED microscopy enables confocal imaging of biological samples with a resolution that is not limited by diffraction. It provides new insights in various fields of biology, such as membrane biology, neurobiology and physiology. Its three dimensional sectioning ability allows the acquisition of high resolution image stacks. Furthermore, STED microscopy enables the recording of dynamic processes and live cell images. We present two-color imaging in confocal STED microscopy with a single STED wavelength. Pulsed and continuous wave lasers in the visible and near infra-red wavelengths range are used for stimulated emission. The resolution enhancement is demonstrated in comparison to confocal imaging with biological specimens.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Hilmar Gugel ; Arnold Giske ; Marcus Dyba and Jochen Sieber
"Confocal imaging at the nanoscale with two-color STED microscopy", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050X (February 28, 2011); doi:10.1117/12.874779; http://dx.doi.org/10.1117/12.874779


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