Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

A dark-field scanning spectroscopy platform for localized scatter and fluorescence imaging of tissue

[+] Author Affiliations
Venkataramanan Krishnaswamy, Ashley M. Laughney, Keith D. Paulsen, Brian W. Pogue

Dartmouth College (USA)

Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070J (February 11, 2011); doi:10.1117/12.875462
Text Size: A A A
From Conference Volume 7907

  • Biomedical Applications of Light Scattering V
  • Adam P. Wax; Vadim Backman
  • San Francisco, California, USA | January 22, 2011

abstract

Tissue ultra-structure and molecular composition provide native contrast mechanisms for discriminating across pathologically distinct tissue-types. Multi-modality optical probe designs combined with spatially confined sampling techniques have been shown to be sensitive to this type of contrast but their extension to imaging has only been realized recently. A modular scanning spectroscopy platform has been developed to allow imaging localized morphology and molecular contrast measures in breast cancer surgical specimens. A custom designed dark-field telecentric scanning spectroscopy system forms the core of this imaging platform. The system allows imaging localized elastic scatter and fluorescence measures over fields of up to 15 mm x 15 mm at 100 microns resolution in tissue. Results from intralipid and blood phantom measurements demonstrate the ability of the system to quantify localized scatter parameters despite significant changes in local absorption. A co-registered fluorescence spectroscopy mode is also demonstrated in a protophorphyrin-IX phantom.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Venkataramanan Krishnaswamy ; Ashley M. Laughney ; Keith D. Paulsen and Brian W. Pogue
"A dark-field scanning spectroscopy platform for localized scatter and fluorescence imaging of tissue", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070J (February 11, 2011); doi:10.1117/12.875462; http://dx.doi.org/10.1117/12.875462


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.