Rapid analysis of white blood cells with diffraction imaging flow cytometry

Xin-Hua Hu; Jun Q. Lu; Yuanming Feng

doi:10.1117/12.876702

11 February 2011 Rapid analysis of white blood cells with diffraction imaging flow cytometry

Xin-Hua Hu, Jun Q. Lu, Yuanming Feng

Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 79070W (2011) https://doi.org/10.1117/12.876702
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

From a foundation of cell optics research program we have recently developed a diffraction imaging flow cytometer method which can be used to rapidly acquire and analyze diffraction image data from single flowing cells. Modeling and experimental investigation of light scattering by white blood cells (WBC) have been conducted which suggest a strong correlation between the fringe patterns related to the texture of the diffraction images and 3D morphological features of the cells. In particular, we present results of calculated and measured diffraction images obtained with three cell lines derived from leukemia patients to demonstrate that texture features of the diffraction images extracted with a gray-scale co-occurrence-matrix algorithm can be used for rapid cell classification.

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Xin-Hua Hu, Jun Q. Lu, and Yuanming Feng "Rapid analysis of white blood cells with diffraction imaging flow cytometry", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070W (11 February 2011); https://doi.org/10.1117/12.876702

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KEYWORDS

Diffraction

Light scattering

3D image processing

Blood

3D image reconstruction

Confocal microscopy

Flow cytometry

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