Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Rapid analysis of white blood cells with diffraction imaging flow cytometry

[+] Author Affiliations
Xin-Hua Hu, Jun Q. Lu

East Carolina Univ. (USA)

Yuanming Feng

Tianjin Univ. (China)

Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070W (February 11, 2011); doi:10.1117/12.876702
Text Size: A A A
From Conference Volume 7907

  • Biomedical Applications of Light Scattering V
  • Adam P. Wax; Vadim Backman
  • San Francisco, California, USA | January 22, 2011

abstract

From a foundation of cell optics research program we have recently developed a diffraction imaging flow cytometer method which can be used to rapidly acquire and analyze diffraction image data from single flowing cells. Modeling and experimental investigation of light scattering by white blood cells (WBC) have been conducted which suggest a strong correlation between the fringe patterns related to the texture of the diffraction images and 3D morphological features of the cells. In particular, we present results of calculated and measured diffraction images obtained with three cell lines derived from leukemia patients to demonstrate that texture features of the diffraction images extracted with a gray-scale co-occurrence-matrix algorithm can be used for rapid cell classification.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Xin-Hua Hu ; Jun Q. Lu and Yuanming Feng
"Rapid analysis of white blood cells with diffraction imaging flow cytometry", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070W (February 11, 2011); doi:10.1117/12.876702; http://dx.doi.org/10.1117/12.876702


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.