Nanoscale nuclear architecture for cancer diagnosis by spatial-domain low-coherence quantitative phase microscopy

Pin Wang; Rajan K. Bista; Walid E. Khalbuss; Wei Qiu; Kevin D. Staton; Lin Zhang; Teresa A. Brentnall M.D.; Randall E. Brand M.D.; Yang Liu

doi:10.1117/12.874693

11 February 2011 Nanoscale nuclear architecture for cancer diagnosis by spatial-domain low-coherence quantitative phase microscopy

Pin Wang, Rajan K. Bista, Walid E. Khalbuss, Wei Qiu, Kevin D. Staton, Lin Zhang, Teresa A. Brentnall M.D., Randall E. Brand M.D., Yang Liu

Author Affiliations +

Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 790704 (2011) https://doi.org/10.1117/12.874693
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Alterations in nuclear architecture are the hallmark diagnostic characteristic of cancer cells. In this work, we show that the nuclear architectural characteristics quantified by spatial-domain low-coherence quantitative phase microscopy (SL-QPM), is more sensitive for the identification of cancer cells than conventional cytopathology. We demonstrated the importance of nuclear architectural characteristics in both an animal model of intestinal carcinogenesis - APC/Min mouse model and human cytology specimens with colorectal cancer by identifying cancer from cytologically noncancerous appearing cells. The determination of nanoscale nuclear architecture using this simple and practical optical instrument is a significant advance towards cancer diagnosis.

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Pin Wang, Rajan K. Bista, Walid E. Khalbuss, Wei Qiu, Kevin D. Staton, Lin Zhang, Teresa A. Brentnall M.D., Randall E. Brand M.D., and Yang Liu "Nanoscale nuclear architecture for cancer diagnosis by spatial-domain low-coherence quantitative phase microscopy", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790704 (11 February 2011); https://doi.org/10.1117/12.874693

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