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Proceedings Article

Optical coherence tomography speckle decorrelation for detecting cell death

[+] Author Affiliations
Golnaz Farhat, Gregory J. Czarnota

Univ. of Toronto (Canada) and Sunnybrook Health Sciences Ctr. (Canada)

Adrian Mariampillai

Ryerson Univ. (Canada) and Ontario Cancer Institute (Canada)

Victor X. D. Yang

Sunnybrook Health Sciences Ctr. (Canada) and Ryerson Univ. (Canada)

Michael C. Kolios

Univ. of Toronto (Canada) and Ryerson Univ. (Canada)

Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790710 (February 11, 2011); doi:10.1117/12.874682
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From Conference Volume 7907

  • Biomedical Applications of Light Scattering V
  • Adam P. Wax; Vadim Backman
  • San Francisco, California, USA | January 22, 2011

abstract

We present a dynamic light scattering technique applied to optical coherence tomography (OCT) for detecting changes in intracellular motion caused by cellular reorganization during apoptosis. We have validated our method by measuring Brownian motion in microsphere suspensions and comparing the measured values to those derived based on particle diffusion calculated using the Einstein-Stokes equation. Autocorrelations of OCT signal intensities acquired from acute myeloid leukemia cells as a function of treatment time demonstrated a significant drop in the decorrelation time after 24 hours of cisplatin treatment. This corresponded with nuclear fragmentation and irregular cell shape observed in histological sections. A similar analysis conducted with multicellular tumor spheroids indicated a shorter decorrelation time in the spheroid core relative to its edges. The spheroid core corresponded to a region exhibiting signs of cell death in histological sections and increased backscatter intensity in OCT images.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Golnaz Farhat ; Adrian Mariampillai ; Victor X. D. Yang ; Gregory J. Czarnota and Michael C. Kolios
"Optical coherence tomography speckle decorrelation for detecting cell death", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790710 (February 11, 2011); doi:10.1117/12.874682; http://dx.doi.org/10.1117/12.874682


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