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Proceedings Article

Cell death monitoring using quantitative optical coherence tomography methods

[+] Author Affiliations
Golnaz Farhat, Gregory J. Czarnota

Univ. of Toronto (Canada) and Sunnybrook Health Sciences Ctr. (Canada)

Victor X. D. Yang

Sunnybrook Health Sciences Ctr. (Canada) and Ryerson Univ. (Canada)

Michael C. Kolios

Univ. of Toronto (Canada) and Ryerson Univ. (Canada)

Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790713 (February 11, 2011); doi:10.1117/12.874721
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From Conference Volume 7907

  • Biomedical Applications of Light Scattering V
  • Adam P. Wax; Vadim Backman
  • San Francisco, California, USA | January 22, 2011

abstract

Cell death is characterized by a series of predictable morphological changes, which modify the light scattering properties of cells. We present a multi-parametric approach to detecting changes in subcellular morphology related to cell death using optical coherence tomography (OCT). Optical coherence tomography data were acquired from acute myeloid leukemia (AML) cells undergoing apoptosis over a period of 48 hours. Integrated backscatter (IB) and spectral slope (SS) were computed from OCT backscatter spectra and statistical parameters were extracted from a generalized gamma (GG) distribution fit to OCT signal intensity histograms. The IB increased by 2-fold over 48 hours with significant increases observed as early as 4 hours. The SS increased in steepness by 2.5-fold with significant changes at 12 hours, while the GG parameters were sensitive to apoptotic changes at 24 to 48 hours. Histology slides indicated nuclear condensation and fragmentation at 24 hours, suggesting the late scattering changes could be related to nuclear structure. A second series of measurements from AML cells treated with cisplatin, colchicine or ionizing radiation suggested that the GG parameters could potentially differentiate between modes of cell death. Distinct cellular morphology was observed in histology slides obtained from cells treated under each condition.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Golnaz Farhat ; Victor X. D. Yang ; Michael C. Kolios and Gregory J. Czarnota
"Cell death monitoring using quantitative optical coherence tomography methods", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790713 (February 11, 2011); doi:10.1117/12.874721; http://dx.doi.org/10.1117/12.874721


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