Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Development of optical immunosensors and their application to the analysis of human bone morphogenetic protein-7 (BMP-7)

[+] Author Affiliations
Chun-Kwang Kim, Jong Il Rhee

Chonnam National Univ. (Korea, Republic of)

Ok-Jae Sohn

B&P Tech Co., Ltd. (Korea, Republic of)

Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080W (February 11, 2011); doi:10.1117/12.873286
Text Size: A A A
From Conference Volume 7908

  • Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
  • Alexander N. Cartwright; Dan V. Nicolau
  • San Francisco, California, USA | January 22, 2011

abstract

In this study, a few optical immunosensors were developed to determine the concentration of BMP-7. Hydrophilic CdSe/ZnS quantum dots (QDs) were synthesized and conjugated to the antibody of BMP-7 (BMP-7Ab). The QDconjugated BMP-7Ab was used as a fluorescence probe at excitation and emission wavelengths of 470 nm and 585 nm, respectively. It was immobilized either on the bottom of the well of a 96-well microtiter plate or on the tip of an optical fiber. Two immunoassays, i.e. the direct and sandwich assays, were studied for their sensitivity. The sensitivity of the direct immunoassay was 1296.21, compared to 384.69 for the sandwich assay. The linear detection range was 0.0-1.0 ng/mL for both assays. Based on the results of the microtiter plate technique, the direct assay technique was used for the development of an optical fiber immunosensor. The optical fiber immunosensor has a linear detection range between 0.0 and 10.0 ng/mL with a detection limit of 0.413 ng/mL. The optical fiber immunosensor was applied to the sequential injection analysis for the automatic determination of BMP-7.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Chun-Kwang Kim ; Jong Il Rhee and Ok-Jae Sohn
"Development of optical immunosensors and their application to the analysis of human bone morphogenetic protein-7 (BMP-7)", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080W (February 11, 2011); doi:10.1117/12.873286; http://dx.doi.org/10.1117/12.873286


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.