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Proceedings Article

Non-radiative excitation fluorescence: driving biology beyond the diffraction limit

[+] Author Affiliations
Pascale Winckler, Rodolphe Jaffiol, Jérôme Plain

Lab. de Nanotechnologie et d'Instrumentation Optique, LRC CEA, CNRS, Univ. de Technologie Troyes (France)

Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 790802 (February 04, 2011); doi:10.1117/12.873846
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From Conference Volume 7908

  • Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
  • Alexander N. Cartwright; Dan V. Nicolau
  • San Francisco, California, USA | January 22, 2011

abstract

We propose a new method of local illumination based on the activation of glass substrates, enables us to drive fluorescence investigations on biological sample at nanometric scale. This local fluorescence excitation is achieved through a non-radiative energy transfer. We demonstrate the potentiality of our technique through two biological relevant applications: imaging of the cell adhesion points and fluorescence correlation spectroscopy in a sub-diffracted volume (attoliter range).

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Pascale Winckler ; Rodolphe Jaffiol and Jérôme Plain
"Non-radiative excitation fluorescence: driving biology beyond the diffraction limit", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 790802 (February 04, 2011); doi:10.1117/12.873846; http://dx.doi.org/10.1117/12.873846


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