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Proceedings Article

High-precision three-dimensional position measurement of particles by digital Gabor holography

[+] Author Affiliations
Myung K. Kim, Leo G. Krzewina

Univ. of South Florida (USA)

Mariana C. Potcoava

Colorado School of Mines (USA)

Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 790806 (February 11, 2011); doi:10.1117/12.876249
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From Conference Volume 7908

  • Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
  • Alexander N. Cartwright; Dan V. Nicolau
  • San Francisco, California, USA | January 22, 2011

abstract

A single exposure of digital Gabor holography (DGH) is used for simultaneous three-dimensional measurement of particle position. The particle sample is set up such that its position can be electro-mechanically manipulated using calibrated piezoelectric transducers in both the lateral and axial directions. The central position of the reconstructed image of the particle is determined by low-pass filtering, thresholding, and center-of-mass calculation. We have obtained less than 20 nm resolution in both the lateral and axial directions in a direct and unambiguous manner. The method is applied to calibration of optical trap strength.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Myung K. Kim ; Mariana C. Potcoava and Leo G. Krzewina
"High-precision three-dimensional position measurement of particles by digital Gabor holography", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 790806 (February 11, 2011); doi:10.1117/12.876249; http://dx.doi.org/10.1117/12.876249


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