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Proceedings Article

Time-resolved and steady-state FRET spectroscopy on commercial biocompatible quantum dots

[+] Author Affiliations
D. Wegner, D. Geißler, H.-G. Löhmannsröben

Univ. Potsdam (Germany)

S. Stufler

Fraunhofer Institute for Applied Polymer Research (Germany)

N. Hildebrandt

Univ. Paris-Sud 11 (France)

Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 79090D (February 11, 2011); doi:10.1117/12.874760
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From Conference Volume 7909

  • Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI
  • Wolfgang J. Parak; Kenji Yamamoto; Marek Osinski
  • San Francisco, California, USA | January 22, 2011

abstract

Semiconductor nanocrystals (quantum dots - QDs) possess unique photophysical properties that make them highly interesting for many biochemical applications. Besides their common use as fluorophores in conventional spectroscopy and microscopy, QDs are well-suited for studying Förster resonance energy transfer (FRET). Size-dependent broadband absorption and narrow emission bands offer several advantages for the use of QDs both as FRET donors and acceptors. QD-based FRET pairs can be efficiently used as biological and chemical sensors for highly sensitive multiplexed detection. In this contribution we present the use of several commercially available QDs (Qdot® Nanocrystals - Invitrogen) as FRET donors in combination with commercial organic dyes as FRET acceptors. In order to investigate the FRET process within our donor-acceptor pairs, we used biotinylated QDs and streptavidin-labeled dyes. The well-known biotinstreptavidin molecular recognition enables effective FRET from QDs to dye molecules and provides defined distances between donor and acceptor. Steady-state and time-resolved fluorescence measurements were performed in order to investigate QD-to-dye FRET. Despite a thick polymer shell around the QDs, our results demonstrate the potential of these QDs as efficient donors both for steady-state and time-resolved FRET applications in nano-biotechnology.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

D. Wegner ; D. Geißler ; S. Stufler ; H.-G. Löhmannsröben and N. Hildebrandt
"Time-resolved and steady-state FRET spectroscopy on commercial biocompatible quantum dots", Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 79090D (February 11, 2011); doi:10.1117/12.874760; http://dx.doi.org/10.1117/12.874760


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