Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

State of the art toxicological and microscopic assessment of biomedical nanocrystals on the lung in vitro

[+] Author Affiliations
Martin J. D. Clift, Peter Gehr, Barbara Rothen-Rutishauser

Univ. Bern (Switzerland)

Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 790909 (February 04, 2011); doi:10.1117/12.875369
Text Size: A A A
From Conference Volume 7909

  • Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI
  • Wolfgang J. Parak; Kenji Yamamoto; Marek Osinski
  • San Francisco, California, USA | January 22, 2011

abstract

Due to the ever increasing production of nanosized materials for a variety of novel applications (e.g. biomedicine), increased research has focused upon understanding the potential toxicity of these nanomaterials. In order to determine the potential toxicity of nanoparticles (NPs), it is essential that, in parallel to biochemical and toxicological testing, their specific route of uptake (if any), as well as their possible and/or subsequent intracellular localisation is investigated. By using a novel 3D in vitro triple cell co-culture model of the human epithelial airway barrier (containing epithelial cells and macrophages (apical-layer) and dendritic cells (basolateral-layer) which has been shown to mimic the architecture of the lung in vivo , in combination with a novel air-liquid interface cell exposure system, it is possible to mimic the exposure of NPs to the lung in vitro . Using both conventional and state-of-the art toxicological tests, in addition to light, laser scanning (including digital image restoration) and transmission electron microscopy methods, it has been possible to determine the interaction of both fluorescent (specifically designed core-shell nanoparticles with shell-embedded fluorophores for use in biomedicine) and electron dense NPs with the in vitro triple cell co-culture. It has been observed that the material of the engineered NPs (either gold or iron oxide) has no significant influence upon the toxicity and intracellular localisation over time. The results of these studies have shown that despite different compositions, specific NPs intended for use in biomedical applications, when exposed realistically (exposure method/concentration/primary contact cells) cause minimal effects to the lung in vitro .

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Martin J. D. Clift ; Peter Gehr and Barbara Rothen-Rutishauser
"State of the art toxicological and microscopic assessment of biomedical nanocrystals on the lung in vitro", Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 790909 (February 04, 2011); doi:10.1117/12.875369; http://dx.doi.org/10.1117/12.875369


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.