Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Combined near infrared photothermolysis and photodynamic therapy by association of gold nanoparticles and an organic dye

[+] Author Affiliations
Elena S. Tuchina, Valery V. Tuchin

N.G. Chernyshevsky Saratov State Univ. (Russian Federation)

Fulvio Ratto, Paolo Matteini, Francesca Rossi, Roberto Pini

Istituto di Fisica Applicata Nello Carrara (Italy)

Boris N. Khlebtsov, Nikolai G. Khlebtsov

Institute of Biochemistry and Physiology of Plants and Microorganisms (Russian Federation)

Sonia Centi, Franco Fusi

Univ. degli Studi di Firenze (Italy)

Proc. SPIE 7911, Plasmonics in Biology and Medicine VIII, 79111C (February 11, 2011); doi:10.1117/12.875122
Text Size: A A A
From Conference Volume 7911

  • Plasmonics in Biology and Medicine VIII
  • Tuan Vo-Dinh; Joseph R. Lakowicz
  • San Francisco, California, USA | January 22, 2011

abstract

We investigated the combination of near infrared (NIR) photothermolysis and photodynamic therapy against different models of bacteria (S. aureus , S. epidermidis both methicillin susceptible and resistant), in order to discover possible synergistic pathways in the fight against cancer. Photothermolysis was mediated by NIR light absorption from gold nanorods, which were coated with polyethylene glycol to gain biocompatibility and provide for a convenient interface with the bacterial cell walls. At the same time photodynamic therapy was delivered by administration of Indocyanine Green (ICG), whose spectrum of molecular excitation overlaps the plasmonic oscillations of gold nanorods (~ 800 nm). Therefore irradiation with NIR light from a low power diode laser resulted into simultaneous photothermolysis and generation of reactive oxygen species and cytotoxic byproducts of ICG. We assessed the inhibition of the bacterial colony forming ability under different NIR light exposures, and compared the performance of the combined treatment (gold nanorods plus ICG) with the projected addition of the separate treatments (either gold nanorods or ICG). Our preliminary results may originate from the interplay of synergistic and conflicting interactions, which may include e.g. the enhanced intake of cytotoxic species due to permeabilization of the bacterial cell walls, quenching of ICG and modification of the bleaching of ICG due to the noble metal surface.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Elena S. Tuchina ; Fulvio Ratto ; Boris N. Khlebtsov ; Sonia Centi ; Paolo Matteini, et al.
"Combined near infrared photothermolysis and photodynamic therapy by association of gold nanoparticles and an organic dye", Proc. SPIE 7911, Plasmonics in Biology and Medicine VIII, 79111C (February 11, 2011); doi:10.1117/12.875122; http://dx.doi.org/10.1117/12.875122


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.