Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Gold nanoparticle mediated cell manipulation using fs and ps laser pulses for cell perforation and transfection

[+] Author Affiliations
D. Heinemann, M. Schomaker, D. Motekaitis, J. Krawinkel, Alexander Heisterkamp

Laser Zentrum Hannover e.V. (Germany) and Excellence Cluster REBIRTH (Germany)

D. Killian, Christian Junghanß

Univ. of Rostock (Germany)

H. M. Escobar

Univ. of Veterinary Medicine Hannover (Germany)

Proc. SPIE 7925, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI, 79250J (February 11, 2011); doi:10.1117/12.874904
Text Size: A A A
From Conference Volume 7925

  • Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI
  • Alexander Heisterkamp; Joseph Neev; Stefan Nolte
  • San Francisco, California, USA | January 22, 2011

abstract

Manipulation of cells requires the delivery of membrane-impermeable substances like genetic materials or proteins into the cytoplasm. Thus delivery of molecules over the cell membrane barrier is one of the key technologies in molecular biology. Many techniques concerning especially the delivery foreign DNA have been developed. Notwithstanding there still is a range of applications where these standard techniques fail to raise the desired results due to low efficiencies, high toxicity or other safety issues. Especially the transfection of sensitive cell types like primary and stem cells can be problematic. Here we present an alternative, laser based technique to perforate the cell membrane and thus allowing efficient delivery of extra cellular molecules: Gold nanoparticles (GNP) are brought into close contact with the cell, were the laser-GNP interaction leads to membrane perforation. This allows the utilisation of a weakly focused laser beam leading to fast scanning of the sample and thus to a high throughput. To investigate the GNP-laser interaction in more detail we have compared membrane perforation obtained by different laser pulse lengths. From our results we assume strong light absorption for ps laser pulses and relatively small particles as the initiating perforation mechanism, whereas an enhanced near field scattering occurs at 200 nm GNP when using fs laser pulses. SEM and ESEM imaging were applied to give a deeper insight in the GNP-cell interaction and the effects of laser radiation on the GNP. Additionally dextran- FITC derivatives of varying sizes were used to investigate the impact of molecule size on delivery efficiency.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

D. Heinemann ; M. Schomaker ; D. Motekaitis ; J. Krawinkel ; D. Killian, et al.
"Gold nanoparticle mediated cell manipulation using fs and ps laser pulses for cell perforation and transfection", Proc. SPIE 7925, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI, 79250J (February 11, 2011); doi:10.1117/12.874904; http://dx.doi.org/10.1117/12.874904


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.