Development path and current status of the NANIVID: a new device for cancer cell studies

Waseem Khan Raja; Michael R. Padgen; James K. Williams; Jeffrey Wyckoff; John Condeelis; James Castracane

doi:10.1117/12.883532

14 February 2011 Development path and current status of the NANIVID: a new device for cancer cell studies

Waseem Khan Raja, Michael R. Padgen, James K. Williams, Jeffrey Wyckoff, John Condeelis, James Castracane

Proceedings Volume 7929, Microfluidics, BioMEMS, and Medical Microsystems IX; 79290A (2011) https://doi.org/10.1117/12.883532
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

Cancer cells create a unique microenvironment in vivo which enables migration to distant organs. To better understand the tumor microenvironment, special tools and devices are required to monitor the interactions between different cell types and the effects of particular chemical gradients. This study presents the design and optimization of a new, versatile chemotaxis device called the NANIVID (NANo IntraVital Device). The device is fabricated using BioMEMS techniques and consists of etched and bonded Pyrex substrates, a soluble factor reservoir, fluorescent tracking beads and a microelectrode array for cell quantification. The reservoir contains a customized hydrogel blend loaded with EGF which diffuses out of the hydrogel to create a chemotactic gradient. This reservoir sustains a steady release of growth factor into the surrounding environment for many hours and establishes a concentration gradient that attracts specific cells to the device. In addition to a cell collection tool, the NANIVID can be modified to act as a delivery vehicle for the local generation of alternate soluble factor gradients to initiate controlled changes to the microenvironment such as hypoxia, ECM stiffness and etc. The focus of this study is to design and optimize the new device for wide ranging studies of breast cancer cell dynamics in vitro and ultimately, implantation for in vivo work.

Citation Download Citation

Waseem Khan Raja, Michael R. Padgen, James K. Williams, Jeffrey Wyckoff, John Condeelis, and James Castracane "Development path and current status of the NANIVID: a new device for cancer cell studies", Proc. SPIE 7929, Microfluidics, BioMEMS, and Medical Microsystems IX, 79290A (14 February 2011); https://doi.org/10.1117/12.883532

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