A unified approach for high throughput analysis of real-time biomolecular interactions in surface plasmon resonance and fluorescence imaging

Catalin Fetita; Nicolas François; Françoise Prêteux; Hervé Delacroix

doi:10.1117/12.877597

9 March 2011 A unified approach for high throughput analysis of real-time biomolecular interactions in surface plasmon resonance and fluorescence imaging

Catalin Fetita, Nicolas François, Françoise Prêteux, Hervé Delacroix

Author Affiliations +

Proceedings Volume 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging; 79651A (2011) https://doi.org/10.1117/12.877597
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

The analysis of real-time biomolecular interactions (observation is performed as the biological interaction occurs) provides information on the formation of target/probe complexes, particularly on their dynamic behaviours. Namely, it allows the determination of the affinity constant, a static value that characterizes the interaction properties, using two dynamic values, the association and dissociation constants. Such dynamic behaviour can be assessed either with surface plasmon resonance (SPR) or uorescence-based biosensors. The challenging issue is the automatic extraction and analysis of the interaction signal for each spotted probe on the biosensor in a highthroughput framework (hundreds of probes). This paper addresses such issue and develops a uniffied approach for analyzing the image data provided by the above-mentioned technologies. A mathematical modelling of the image data allowed building-up a virtual biosensor able to simulate biologic experiences related to various possible parameters (level of signal and noise, presence of artefacts, surface functionalization, spotting heterogeneity). Based on such simulation, a generic and automated approach combining 3D mathematical morphology and spatio-temporal classiffication is proposed for detecting the interacting probes, segmenting the regions of effective signal, and characterizing the associated affinity constants. The developed method has been assessed both qualitatively and quantitatively on simulated and experimental datasets and showed accurate results (maximum error of 7% for the most difficult cases in terms of noise and surface functionalization).

Citation Download Citation

Catalin Fetita, Nicolas François, Françoise Prêteux, and Hervé Delacroix "A unified approach for high throughput analysis of real-time biomolecular interactions in surface plasmon resonance and fluorescence imaging", Proc. SPIE 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, 79651A (9 March 2011); https://doi.org/10.1117/12.877597

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