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Proceedings Article

Determining biomolecular structures by time-resolved fluorescence

[+] Author Affiliations
Olaf J. Rolinski, Aaron Hernandez-Santana, Duncan Graham

Univ. of Strathclyde (United Kingdom)

Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472N (October 27, 2006); doi:10.1117/12.710984
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From Conference Volume 6047

  • Fourth International Conference on Photonics and Imaging in Biology and Medicine
  • Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin
  • Tianjin, China | September 03, 2005

abstract

We demonstrate a new fluorescence resonance energy transfer (FRET) based approach to determine the donor-acceptor distributions and apply it to two model molecular systems: double stranded DNA labeled with Hoechst 33258 and FAM, and perylene randomly surrounded by cobalt ions in a bulk solution. The approach makes some generic assumptions regarding the FRET kinetics, but no a priori assumptions regarding the distribution function.

© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Olaf J. Rolinski ; Aaron Hernandez-Santana and Duncan Graham
"Determining biomolecular structures by time-resolved fluorescence", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472N (October 27, 2006); doi:10.1117/12.710984; http://dx.doi.org/10.1117/12.710984


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