A role for Nrf2 in UVA-mediated heme oxygenase induction and protection from membrane damage in human skin fibroblasts

Haibin Li; Linhao Li; Linhong Deng; Gurinder Singh; Rex M. Tyrrell; J. Li Zhong

doi:10.1117/12.870980

8 November 2010 A role for Nrf2 in UVA-mediated heme oxygenase induction and protection from membrane damage in human skin fibroblasts

Haibin Li, Linhao Li, Linhong Deng, Gurinder Singh, Rex M. Tyrrell, J. Li Zhong

Author Affiliations +

Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78450X (2010) https://doi.org/10.1117/12.870980
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Our previous study has shown that Ultraviolet-A (UVA) irradiation induces heme oxygenase 1 (HO-1) expression in cultured human primary skin fibroblasts FEK4. In the present study, we demonstrate a coordinated induction of HO-1 and NF-E2-related factor 2 (Nrf2) following UVA irradiation or hemin treatment. The induction of HO-1 by either UVA irradiation or hemin treatment was largely abolished by down-regulation of Nrf2 with its targeted short interfering RNA (siNrf2). The study further reveals that knockdown of Nrf2 protein increased UVA-induced cell death measured by MTS assay. These findings together indicate that Nrf2-mediated induction of HO-1 expression may provide a cytoprotection for human skin cells from oxidative damage.

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Haibin Li, Linhao Li, Linhong Deng, Gurinder Singh, Rex M. Tyrrell, and J. Li Zhong "A role for Nrf2 in UVA-mediated heme oxygenase induction and protection from membrane damage in human skin fibroblasts", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78450X (8 November 2010); https://doi.org/10.1117/12.870980

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KEYWORDS

Skin

Proteins

Cell death

Lithium

Chemical elements

Defense and security

Modulation

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