Fluorescence rejection by shifted excitation Raman difference spectroscopy

Wenlong Zou; Zhijian Cai; Jianhong Wu

doi:10.1117/12.869893

11 November 2010 Fluorescence rejection by shifted excitation Raman difference spectroscopy

Wenlong Zou, Zhijian Cai, Jianhong Wu

Proceedings Volume 7855, Optical Metrology and Inspection for Industrial Applications; 78551M (2010) https://doi.org/10.1117/12.869893
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Raman spectroscopy is often hampered by strong fluorescence background that can easily bury the much weaker Raman signal. One of the most widely used techniques to reject the fluorescence disturbance is the shifted-excitation Raman difference spectroscopy (SERDS), which incorporates multiple wavelengths as excitation sources. This paper proposed a SERDS system with 532 nm and 526.5 nm DPSS lasers and a home-made holographic notch filter. In this system, two lasers illuminate the sample alternatively, and two series of spectra are acquired to generate the difference spectrum. And then a regularization constrained least square algorithm is proposed to reconstruct the conventional Raman spectrum from the difference spectrum. Computer simulation of this algorithm is provided and the preliminary experimental results are presented. It demonstrates that this system can effectively reject the fluorescence.

Citation Download Citation

Wenlong Zou, Zhijian Cai, and Jianhong Wu "Fluorescence rejection by shifted excitation Raman difference spectroscopy", Proc. SPIE 7855, Optical Metrology and Inspection for Industrial Applications, 78551M (11 November 2010); https://doi.org/10.1117/12.869893

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available