Assessing topographic cutaneous autofluorescence variation using fluorescence UV and visible excitation emission matrix (EEM) spectroscopy

Jianhua Zhao; Soodabeh Zandi; Florina Feng; Haishan Zeng; David I. McLean; Harvey Lui

doi:10.1117/12.878770

17 February 2011 Assessing topographic cutaneous autofluorescence variation using fluorescence UV and visible excitation emission matrix (EEM) spectroscopy

Jianhua Zhao, Soodabeh Zandi, Florina Feng, Haishan Zeng, David I. McLean, Harvey Lui

Author Affiliations +

Proceedings Volume 7883, Photonic Therapeutics and Diagnostics VII; 78830I (2011) https://doi.org/10.1117/12.878770
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Cutaneous autofluorescence properties were systematically studied using fluorescence excitation emission matrix spectroscopy. Twenty-six healthy subjects with a mean age of 34 (range 21-74) participated in this study. The fluorescence of major skin fluorophores such as tryptophan, collagen, elastin and NADH could be readily identified. On average, facial skin shows strong tryptophan and measurable porphyrin fluorescence; the palm and nail show strong tryptophan and keratin fluorescence. These results demonstrate that regional topographic variations exist not only in the amount of fluorescence but also in the relative distribution of fluorophores in normal skin. Moreover this provides a basis for future interpretation of autofluorescence in diseased skin.

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Jianhua Zhao, Soodabeh Zandi, Florina Feng, Haishan Zeng, David I. McLean, and Harvey Lui "Assessing topographic cutaneous autofluorescence variation using fluorescence UV and visible excitation emission matrix (EEM) spectroscopy", Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78830I (17 February 2011); https://doi.org/10.1117/12.878770

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