Fluorescence-based calculus detection using a 405-nm excitation wavelength

O. Brede; F. Schelle; S. Krueger; B. Oehme; C. Dehn; M. Frentzen; A. Braun

doi:10.1117/12.873730

17 February 2011 Fluorescence-based calculus detection using a 405-nm excitation wavelength

O. Brede, F. Schelle, S. Krueger, B. Oehme, C. Dehn, M. Frentzen, A. Braun

Proceedings Volume 7884, Lasers in Dentistry XVII; 788405 (2011) https://doi.org/10.1117/12.873730
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The aim of this study was to assess the difference of fluorescence signals of cement and calculus using a 405 nm excitation wavelength. A total number of 20 freshly extracted teeth was used. The light source used for this study was a blue LED with a wavelength of 405nm. For each tooth the spectra of calculus and cementum were measured separately. Fluorescence light was collimated into an optical fibre and spectrally analyzed using an echelle spectrometer (aryelle 200, Lasertechnik Berlin, Germany) with an additionally bandpass (fgb 67, Edmund Industrial Optics, Karlsruhe, Germany). From these 40 measurements the median values were calculated over the whole spectrum, leading to two different median spectra, one for calculus and one for cementum. For further statistical analysis we defined 8 areas of interest (AOI) in wavelength regions, showing remarkable differences in signal strength. In 7 AOIs the intensity of the calculus spectrum differed statistically significant from the intensity of the cementum spectrum (p < 0.05). A spectral difference could be shown between calculus and cement between 600nm and 700nm. Thus, we can conclude that fluorescence of calculus shows a significant difference to the fluorescence of cement. A differentiation over the intensity is possible as well as over the spectrum. Using a wavelength of 405nm, it is possible to distinguish between calculus and cement. These results could be used for further devices to develop a method for feedback controlled calculus removal.

Citation Download Citation

O. Brede, F. Schelle, S. Krueger, B. Oehme, C. Dehn, M. Frentzen, and A. Braun "Fluorescence-based calculus detection using a 405-nm excitation wavelength", Proc. SPIE 7884, Lasers in Dentistry XVII, 788405 (17 February 2011); https://doi.org/10.1117/12.873730

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