We recently developed several versions of a multimodal adaptive optics (AO) retinal imager, which includes highresolution scanning laser ophthalmoscopy (SLO) and Fourier domain optical coherence tomography (FDOCT) imaging channels as well as an auxiliary wide-field line scanning ophthalmoscope (LSO). Some versions have also been equipped with a fluorescence channel and a retinal tracker. We describe the performance of three key features of the multimodal AO system including: simultaneous SLO/OCT imaging, which allows SLO/OCT co-registration; a small animal imaging port, which adjusts the beam diameter at the pupil from 7.5 to 2.5 mm for use with small animals ubiquitous in biological research or for extended depth-of-focus imaging in humans; and slow scan Doppler flowmetry imaging using the wide field auxiliary LSO imaging channel. The systems are currently deployed in several ophthalmology clinics and research laboratories and several investigations have commenced on patients with a variety of retinal diseases and animals in vision research.© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.