Confocal Raman microscopy for identification of bacterial species in biofilms

Brooke D. Beier; Robert G. Quivey; Andrew J. Berger

doi:10.1117/12.871819

8 February 2011 Confocal Raman microscopy for identification of bacterial species in biofilms

Brooke D. Beier, Robert G. Quivey, Andrew J. Berger

Proceedings Volume 7888, Frontiers in Biological Detection: From Nanosensors to Systems III; 78880D (2011) https://doi.org/10.1117/12.871819
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Implemented through a confocal microscope, Raman spectroscopy has been used to distinguish between biofilm samples of two common oral bacteria species, Streptococcus sanguinis and mutans, which are associated with healthy and cariogenic plaque, respectively. Biofilms of these species are studied as a model of dental plaque. A prediction model has been calibrated and validated using pure biofilms. This model has been used to identify the species of transferred and dehydrated samples (much like a plaque scraping) as well as hydrated biofilms in situ. Preliminary results of confocal Raman mapping of species in an intact two-species biofilm will be shown.

Citation Download Citation

Brooke D. Beier, Robert G. Quivey, and Andrew J. Berger "Confocal Raman microscopy for identification of bacterial species in biofilms", Proc. SPIE 7888, Frontiers in Biological Detection: From Nanosensors to Systems III, 78880D (8 February 2011); https://doi.org/10.1117/12.871819

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