Performance of line-scanning confocal microscopy in human skin: investigation of potential for clinical translation

Bjorg Larson; Gary Peterson; Sanjeewa Abeytunge; Milind Rajadhyaksha

doi:10.1117/12.876171

21 February 2011 Performance of line-scanning confocal microscopy in human skin: investigation of potential for clinical translation

Bjorg Larson, Gary Peterson, Sanjeewa Abeytunge, Milind Rajadhyaksha

Author Affiliations +

Proceedings Volume 7890, Advanced Biomedical and Clinical Diagnostic Systems IX; 78900O (2011) https://doi.org/10.1117/12.876171
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Line-scanning, using 8-10 optical components, linear-array detectors and custom-FPGA electronics, may enable smaller, simpler and lower-cost confocal microscopes to accelerate translation to the clinic. The adaptability of commercially available low-cost array detectors for confocal microscopy is being investigated. Measurements of optical sectioning and lateral resolution showed good agreement with theory, and are comparable to that of point-scanning systems. LSFs through full thickness of human epidermis show a two-fold degradation in sectioning performance. Imaging of human epidermis in vivo demonstrates nuclear and cellular detail down to the basal layer with a bench top setup and also a compact clinical prototype. Blood flow in oral mucosa can be imaged using the clinical prototype. However, speckle and background noise degrade contrast and resolution of the image.

Citation Download Citation

Bjorg Larson, Gary Peterson, Sanjeewa Abeytunge, and Milind Rajadhyaksha "Performance of line-scanning confocal microscopy in human skin: investigation of potential for clinical translation", Proc. SPIE 7890, Advanced Biomedical and Clinical Diagnostic Systems IX, 78900O (21 February 2011); https://doi.org/10.1117/12.876171

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