Hyperspectral imaging system for imaging O2Hb and HHb concentration changes in tissue for various clinical applications

John H. G. M. Klaessens; Rowland de Roode; Rudolf M. Verdaasdonk; Herke Jan Noordmans

doi:10.1117/12.875110

21 February 2011 Hyperspectral imaging system for imaging O₂Hb and HHb concentration changes in tissue for various clinical applications

John H. G. M. Klaessens, Rowland de Roode, Rudolf M. Verdaasdonk, Herke Jan Noordmans

Author Affiliations +

Proceedings Volume 7890, Advanced Biomedical and Clinical Diagnostic Systems IX; 78900R (2011) https://doi.org/10.1117/12.875110
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

To observe local variations in temperature, oxygenation and blood perfusion over time, four imaging systems were developed and compared: Two systems consisting of white broadband light source and a CCD camera in combination with a Liquid Crystal Tunable Filter, one in the visual domain, 420-730 nm, and one in the infrared domain, 650-1100 nm. Thirdly, a CCD camera in combination with a software controlled hyper-spectral light source consisting of a panel with 600 LEDs divided in 17 spectral groups in the range from 370 to 880 nm so that specific spectral distributions can be generated at high repetition rate (>1000 Hz) and, fourthly a standard IR thermal camera for comparison. From the acquired images at the selected wavelengths chromophores concentration images of oxy and deoxy hemoglobin can be calculated applying different algorithms. These imaging techniques were applied and compared for various clinical applications: Tumor demarcation, early inflammation, effectiveness of peripheral nerve block anesthesia, and localization of epileptic seizure. The relative changes in oxygenation and temperature could be clearly observed in good correlation with the physiological condition. The algorithms and data collection/processing can be optimized to enable a real-time diagnostic technique.

Citation Download Citation

John H. G. M. Klaessens, Rowland de Roode, Rudolf M. Verdaasdonk, and Herke Jan Noordmans "Hyperspectral imaging system for imaging O₂Hb and HHb concentration changes in tissue for various clinical applications", Proc. SPIE 7890, Advanced Biomedical and Clinical Diagnostic Systems IX, 78900R (21 February 2011); https://doi.org/10.1117/12.875110

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available