Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy

Yuan Luo; Ioannis Zervantonakis; Se Baek Oh; Roger Kamm; George Barbastathis

doi:10.1117/12.874570

22 February 2011 Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy

Yuan Luo, Ioannis Zervantonakis, Se Baek Oh, Roger Kamm, George Barbastathis

Proceedings Volume 7891, Design and Quality for Biomedical Technologies IV; 78910C (2011) https://doi.org/10.1117/12.874570
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.

Citation Download Citation

Yuan Luo, Ioannis Zervantonakis, Se Baek Oh, Roger Kamm, and George Barbastathis "Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy", Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 78910C (22 February 2011); https://doi.org/10.1117/12.874570

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