Low-light hyperspectral imager for characterization of biological samples based on an sCMOS image sensor

J. Hernandez-Palacios; L. L. Randeberg; I. J Haug; I. Baarstad; T. Løke; T. Skauli

doi:10.1117/12.875770

22 February 2011 Low-light hyperspectral imager for characterization of biological samples based on an sCMOS image sensor

J. Hernandez-Palacios, L. L. Randeberg, I. J Haug, I. Baarstad, T. Løke, T. Skauli

Author Affiliations +

Proceedings Volume 7891, Design and Quality for Biomedical Technologies IV; 78910V (2011) https://doi.org/10.1117/12.875770
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The new "scientific CMOS" (sCMOS) sensor technology has been tested for use in hyperspectral imaging. The sCMOS offers extremely low readout noise combined with high resolution and high speed, making it attractive for hyperspectral imaging applications. A commercial HySpex hyperspectral camera has been modified to be used in low light conditions integrating an sCMOS sensor array. Initial tests of fluorescence imaging in challenging light settings have been performed. The imaged objects are layered phantoms labelled with controlled location and concentration of fluorophore. The camera has been compared to a state of the art spectral imager based on CCD technology. The image quality of the sCMOS-based camera suffers from artifacts due to a high density of pixels with excessive noise, attributed to the high operating temperature of the array. Image processing results illustrate some of the benefits and challenges of the new sCMOS technology.

Citation Download Citation

J. Hernandez-Palacios, L. L. Randeberg, I. J Haug, I. Baarstad, T. Løke, and T. Skauli "Low-light hyperspectral imager for characterization of biological samples based on an sCMOS image sensor", Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 78910V (22 February 2011); https://doi.org/10.1117/12.875770

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